Pad tokenize

Tokenize and pad a batch of data for scGPT integration zero-shot inference or fine-tuning

Info

ID: pad_tokenize
Namespace: scgpt

Example commands

You can run the pipeline using nextflow run.

View help

You can use --help as a parameter to get an overview of the possible parameters.

nextflow run openpipelines-bio/openpipeline \
  -r 2.1.0 -latest \
  -main-script target/nextflow/scgpt/pad_tokenize/main.nf \
  --help

Run command

Example of params.yaml 
# Inputs
input: # please fill in - example: "input.h5mu"
modality: "rna"
model_vocab: # please fill in - example: "vocab.json"
# var_gene_names: "foo"
var_input: "id_in_vocab"
input_obsm_binned_counts: "binned_counts"

# Outputs
# output: "$id.$key.output.h5mu"
# output_compression: "gzip"
obsm_gene_tokens: "gene_id_tokens"
obsm_tokenized_values: "values_tokenized"
obsm_padding_mask: "padding_mask"

# Arguments
pad_token: "<pad>"
pad_value: -2
# max_seq_len: 123

# Nextflow input-output arguments
publish_dir: # please fill in - example: "output/"
# param_list: "my_params.yaml"
nextflow run openpipelines-bio/openpipeline \
  -r 2.1.0 -latest \
  -profile docker \
  -main-script target/nextflow/scgpt/pad_tokenize/main.nf \
  -params-file params.yaml
Note

Replace -profile docker with -profile podman or -profile singularity depending on the desired backend.

Argument groups

Inputs

Name Description Attributes
--input The input h5mu file of pre-processed data. file, required, example: "input.h5mu"
--modality string, default: "rna"
--model_vocab Path to model vocabulary file. file, required, example: "vocab.json"
--var_gene_names The name of the .var column containing gene names. When no gene_name_layer is provided, the .var index will be used. string
--var_input The name of the adata.var column containing boolean mask for vocabulary-cross checked and/or highly variable genes. string, default: "id_in_vocab"
--input_obsm_binned_counts The name of the .obsm field containing the binned counts to be padded and tokenized. string, default: "binned_counts"

Outputs

Name Description Attributes
--output The output h5mu file containing obsm arrays for gene tokens, tokenized data and padding mask. file, required, example: "output.h5mu"
--output_compression The compression type for the output file. string, example: "gzip"
--obsm_gene_tokens The key of the .obsm array containing the gene token ids string, default: "gene_id_tokens", example: "values.pt"
--obsm_tokenized_values The key of the .obsm array containing the count values of the tokenized genes string, default: "values_tokenized"
--obsm_padding_mask The key of the .obsm array containing the padding mask. string, default: "padding_mask"

Arguments

Name Description Attributes
--pad_token Token used for padding. string, default: "<pad>"
--pad_value The value of the padding token. integer, default: -2
--max_seq_len The maximum sequence length of the tokenized data. Defaults to the number of features if not provided. integer

Authors

  • Dorien Roosen (maintainer, author)

  • Elizabeth Mlynarski (author)

  • Weiwei Schultz (contributor)