Cellranger count

Align fastq files using Cell Ranger count.

Info

ID: cellranger_count
Namespace: mapping

Links

Source

Example commands

You can run the pipeline using nextflow run.

View help

You can use --help as a parameter to get an overview of the possible parameters.

nextflow run openpipelines-bio/openpipeline \
  -r 1.0.1 -latest \
  -main-script target/nextflow/mapping/cellranger_count/main.nf \
  --help

Run command

Example of params.yaml

# Inputs
input: # please fill in - example: ["sample_S1_L001_R1_001.fastq.gz", "sample_S1_L001_R2_001.fastq.gz"]
reference: # please fill in - example: "reference.tar.gz"

# Outputs
# output: "$id.$key.output.output"

# Arguments
# expect_cells: 3000
chemistry: "auto"
secondary_analysis: false
generate_bam: true
include_introns: true

# Nextflow input-output arguments
publish_dir: # please fill in - example: "output/"
# param_list: "my_params.yaml"

nextflow run openpipelines-bio/openpipeline \
  -r 1.0.1 -latest \
  -profile docker \
  -main-script target/nextflow/mapping/cellranger_count/main.nf \
  -params-file params.yaml

Note

Replace -profile docker with -profile podman or -profile singularity depending on the desired backend.

Argument groups

Inputs

Name	Description	Attributes
--input	The fastq.gz files to align. Can also be a single directory containing fastq.gz files.	List of file, required, example: "sample_S1_L001_R1_001.fastq.gz", "sample_S1_L001_R2_001.fastq.gz", multiple_sep: ";"
--reference	The path to Cell Ranger reference tar.gz file. Can also be a directory.	file, required, example: "reference.tar.gz"

Outputs

Name	Description	Attributes
--output	The folder to store the alignment results.	file, required, example: "/path/to/output"

Arguments

Name	Description	Attributes
--expect_cells	Expected number of recovered cells, used as input to cell calling algorithm.	integer, example: 3000
--chemistry	Assay configuration. - auto: autodetect mode - threeprime: Single Cell 3’ - fiveprime: Single Cell 5’ - SC3Pv1: Single Cell 3’ v1 - SC3Pv2: Single Cell 3’ v2 - SC3Pv3: Single Cell 3’ v3 - SC3Pv3LT: Single Cell 3’ v3 LT - SC3Pv3HT: Single Cell 3’ v3 HT - SC5P-PE: Single Cell 5’ paired-end - SC5P-R2: Single Cell 5’ R2-only - SC-FB: Single Cell Antibody-only 3’ v2 or 5’ See https://kb.10xgenomics.com/hc/en-us/articles/115003764132-How-does-Cell-Ranger-auto-detect-chemistry- for more information.	string, default: "auto"
--secondary_analysis	Whether or not to run the secondary analysis e.g. clustering.	boolean, default: FALSE
--generate_bam	Whether to generate a BAM file.	boolean, default: TRUE
--include_introns	Include intronic reads in count (default=true unless –target-panel is specified in which case default=false)	boolean, default: TRUE

Authors

• Angela Oliveira Pisco (author)

• Samuel D’Souza (author)

• Robrecht Cannoodt (author, maintainer)